Defining Menin Binding Partners in Ewing sarcoma

Background

Preliminary data suggests that EWS-FLI1, a pathognomonic translocation of a tumor from Ewing’s sarcoma (ES) on mesenchymal stem cells (MSC) origin, may alter the menin interactome. It has been recently discovered that menin is critical for ES tumorigenicity, however, the binding partners of menin in the context of ES are currently unknown.

Project Goal

Aaron will be working with the lab to identify the menin binding partners in ES and determine whether they are influences by EWS-FIL1. They will do this by performing co-immunoprecipitation to pull-down menin MSC from Ed or MSC. He will be working with the team to use tools and strategies, such as western blots and densitometry, to compare the levels of menin binding partners in the different cellular contexts, as well as collaborate with a proteomics research laboratory to further identify menin binding partners. The end goal of this project is to provide information about distinct signaling pathways and targets driving malignant development in ES with therapeutic potential.